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Warm ischemia time of different human liver cells and the significance of free calcium concentration


Cellular deposition of collagen fibers can be seen well filled; * 136 * 1996 Medical Journal of PLA 『j s 2l Lane 4 plastic Wong Man!! 'L! plastic part of ischemic liver cell mitochondria 45min break. Degranulation of the endoplasmic reticulum, nuclear chromatin margination, Disse inflammatory cell infiltration within the gap; ischemia 6Omin irregular liver cells, some broken or fuzzy, lysosome formation. Schedule of different warm ischemia time of liver cells CCa protein binding stored in the mitochondria, smooth endoplasmic reticulum, the solute free ca 'ground state level of ≤ 10 ~ mol / L modern study found that cell damage to cell death within the ca Warm ischemia time of different activities and thus the intracellular liver (Ca3i dynamics of a cell can be used as biological indicators. Which reflects the liver cells from ischemia during the production of different degree of pathological lesion studies have found that rat liver intracellular f_Ca] i sustained ≥ 10mol / L cells when irreversible damage occurs. The study found that people of different warm ischemia time of liver hepatocytes [ca gradually add to the pathological changes consistent with ischemia time were, cirrhosis related to the situation. both groups of liver cells sensitized blood 30rain [ca) i still 10mo | / L range, the main pathological changes of varying degrees organelle swelling, reversible damage. But the cirrhosis group [Ca'3i higher degree of pathological damage and significantly compared with those without cirrhosis, cirrhosis of the liver has been reflected in tolerance to warm ischemia capacity inferior without cirrhosis persons. When ischemic time greater than 30min after the cirrhosis group [Ca] i abrupt rise to sustained ischemia and 45min dark ≥ 10mol / L, organelle ultrastructure showed intimal rupture, nuclear chromatin margination and other cell early signs of necrosis, ischemia 60rainCCa3i up to 10mol / I, the liver cells showed some nuclear ambiguity even rupture and other serious diseases. without cirrhosis group ischemia 45rain [ca] i still 10mol / I, the ischemic 60min Fonda 10mol / l, than the same period of pathological damage was mild cirrhosis group. tips. without cirrhosis of liver cell membrane pump function were better, if to take certain protective measures to make the cells [ca3i not sustained ≥ l0mol / I, there may be security-resistant by 45 ~ 60min of warm ischemia time. This quantitative indicators from the cell biology for the non-cirrhosis were subjected to ultra-safe time Liver surgery is more complicated to complete the feasibility of clinical practice provide some experimental basis for the cirrhotic liver tissue membrane pump dysfunction, suffered more than 30min ischemic injury that may be due to insufficient supply of energy, membrane pump sexually dysfunctional and there increase the intracellular ca overload, and thus trigger oxygen free radicals, membrane lipid peroxidation damage to the role of free radicals, leading to irreversible cell damage. The latter should be strictly controlled on the warm ischemia time, and take effective measures to reduce the hepatic ischemia in liver cells ca overload. To prevent postoperative liver failure. Improve the success rate of surgery