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Xenotransplantation of microencapsulated rat islets in diabetic mice, experimental observations


Group 5 mice were up 3.3,7.7,12.0,14.5,25.2 body weight, while water intake, urine output decreased. 1 capsule around the island of microencapsulated xenogeneic cells shifted arbitrarily on a ● ■ rhino with a small square of wood 2.3 transplant before and after the morphology of microencapsulated islets were observed under an inverted microscope, showing that pre-transplant APA microcapsules are spherical and smooth surface, uniform size, a diameter of about 250 ~ 400m; each capsule 1 or 2 parcels of islet cell mass. 1 month after transplantation, the mice tested open abdominal cavity, abdominal cavity with normal saline to collect sediment. Microscope, spherical microcapsules with smooth surface, intracapsular islet cell mass wrapped stained orange by DTZ, no obvious capsule surrounding fibrous tissue (Figure 2) 2.4 diabetic mice after transplantation of pancreatic tissue Check the microcapsules group 1 month after transplantation, the mice were killed, pancreatic tissue, HE staining, microscopy, pancreatic necrosis, pancreatic islet cell mass no complete save! ll a j5 ● i · 10O · good good at field z 1 month from small countries of a cavity APA received a large emblem of the coin pouch bag snapped an island (DTZ stain × i00) in the. 3 Discussion islet graft quality and quantity of islet transplantation is to determine the critical success factors. In this study, 0.1 collagenase, to short, repeated several times to digest manner, with a 9Dextran 22 a 1614 density gradient method of purification of islets, islet cell mass obtained purity of Will be implanted in diabetic mice, the rapid decline in blood sugar can 9 ~ 12mmol / L, while the air bag and control group, no significant changes in blood sugar tested mice. That this method can obtain higher purity and vitality of the rat islets. Since the early 8O, Lim and Sun reported use of APA microencapsulated islets for transplantation has been the method used by many researchers and is considered the most potential is one of the immune isolation technology based on poly-lysine as the main material, wrapped in the biocompatibility of alginate to form semi-permeable membrane, which can allow water, glucose, insulin and other small molecules freely, but restrictions and macromolecules such as immunoglobulin by immunocompetent cells, it is the role of immune isolation. The experimental group subjects logo pouch rats decreased blood glucose significantly higher than the duration of cyst group, showing that the immune rejection separation membrane has a good effect. microcapsules key production technology is to control the capsule size and membrane strength and surface smoothness. Capsule capsule is too large may be due to 20 (z, transplant easily deformed and broken. capsule surface roughness, can fibrous tissue surrounding the loss of function. Therefore, the different production methods, can microcapsules very different quality and transplantation logo capsules made of static instrument, can better control the size of the capsule to produce a smooth surface, has a higher quality of emblem of the strength of capsules, cell transplantation in experimental xenograft studies and achieved encouraging results. In this study, microcapsules using SUN's static analyzer and improved production of APA microcapsule production method, according to the size of islet cell mass volume production of the corresponding emblem capsule, reducing the capsule. dead space microscopy showed that islet cell survival, the emblem of capsule surface is smooth, no fibrous tissue surrounded. that we make high quality emblem capsule, which can effectively protect the islet from rejection capsule, did not cause significant tissue reaction. The SUN's experiment with static electricity produced emblem capsule microcapsule production technology, can significantly prolong rat islet xenografts as the survival time in mice, show that the microcapsules have good biocompatibility of the immune function and isolation in order to overcome xenograft tissue rejection in transplantation provides a promising way. (Thanks to Toronto, Canada, Professor Department of Physiology, SUNA proud to dry capsule with the help of productions)