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Posted: Tue 16:00, 18 Jan 2011 Post subject: tory burch shoes Tissue Culture of Hope and Rapid |
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Tissue Culture of Hope and Rapid Propagation
tachyssieboldiiHUYa. Qin, XIAOYa · Ping (CollegeofLifeScience, ShaanxiNormalUniversity, X / grumble 710062) 1 plant names and Ling (Stachyssieboldii). 2 material category rhizomes. 3 initial medium culture conditions: 1/2MS,[link widoczny dla zalogowanych], do not add any hormones. Callus induction medium: (1) MS +6. BA1.2mg · L (units of the same below) + NAA0.6; (2) MS +6. BA1.8 + NAA0.6; (3) MS +6- BA2.4 + NAA0.6; (4) MS +6- BA3 + NAA0.2; (5) MS +6 · BA2 + NAA0.1; ( 6) MS +6. BA2.5 + NAA0.1. Bud induction medium: (7) MS +6, BA2 + KT0.1 + NAA0.1. Proliferation medium: ( MS +6- BA2 + NAA0.2. Callus and shoot induction media were supplemented with 4% sucrose, proliferation medium supplemented with 3% sucrose, agar 0.6% ~ 0.8%,[link widoczny dla zalogowanych], pH5.8. Culture temperature (25 ± 4) oC,[link widoczny dla zalogowanych], illumination for the 1500 ~ 2500lx, light l2 ~ l4h · d ~. 4 growth and differentiation of the acquisition of 4.1 sterile materials and callus induction of young plants were taken back, running water 0.5 ~ 2h, the interception of stems, placed in flasks, 70% ethanol for l5 ~ 20s, 0.1% mercuric chloride for surface disinfection 7 ~ 15min, sterile water for 5 to 6 times, seeded in the starting medium. 7d after inoculation, the sterile stems are switching to (1) to (6) of callus induction medium. 3d after transfer, (5), (6) of the medium on the formation of stem callus; 6d, the other medium on both callus. (5), (6) of callus formation on medium speed. 4.2 buds callus induction will be transferred to shoot inducing medium, 5d,[link widoczny dla zalogowanych], the changes in callus began to grow, 15d after the differentiation of bud out of the formation of adventitious buds, and many buds. 4.3 The robust growth of the proliferation of cultured buds will be divided into 3 to 5 for a small cluster into the proliferation medium for bud proliferation and elongation of training. Plantlets grew to 4 to 6 sections (the growth of 30d or so), nearly clipping the top of each seedling and cut a few sections, re-implantation were cultured in proliferation medium. At this time also a large number of axillary bud differentiation, the proliferation of axillary buds cut received a medium, the proliferation can be buds again. This adapter several times, from the tall stems of plants grow a large number of adventitious roots (Figure 1). 4.4 Domestication and transplanting of plantlets with roots to the domestication of a complete method. Grown in tissue culture medium after the formation of adventitious roots, the culture bottles into plastic canopy, semi-open culture, l5 ~ 20d, directly transferred into the sand bed, and finally to the pot and then transplanting survival rate of transplant up to 98%. 5 is the meaning and spirit of progress in the Soviet Union is a perennial water Labiatae herbs. Stachyose rich, this sugar is a bifidus factor oligosaccharides, is the only class of oligosaccharides in the large number of plants extracted from natural products. Can regulate the human micro-ecological environment, promote calcium absorption, lower cholesterol, high blood pressure,[link widoczny dla zalogowanych], high cholesterol, osteoporosis, constipation, diabetes, tooth decay and other diseases, with good health and medicinal value. Of Hope for the northern half of cultivation in arid and semi arid areas, the current distribution of the area under natural conditions is very small. Using tissue culture method can rapidly expanding area under cultivation, to improve the semi-arid northern China semi-arid areas of the agricultural structure and ecological environment, the formation of regional agriculture, may have significance. Tissue Culture of Hope has not been reported. Figure 1 to spiritual growth and proliferation of adventitious buds and revised Received 2002-05.13 2002-07.18 · E · mail: huyaqln21 @ yahoo. com. cn
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