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Toxoplasma gondii B1 gene by nested PCR Detection


9eE} TT} CeP ∈ E} E Guangdong take T9E} CTTTAe circle 1: Arch Insect Kunshan group fell again pumping capacity of child-PCR DNA sequence comparison with the RH strain sequence of mouth. 2.2 Sensitivity test: will Kunshan Toxoplasma gondii isolates (mouse ascites sediments) with normal saline diluted to 10 times the concentration gradient of 8, then extracted by proteinase K digestion and DNA for nested PCR and common PCR (ie Law the sets of primers PcR) amplification, and the results shown in Table 2. 2 children wrapped in bow-PCR group insects mantis power compared with ordinary PCR in peripheral blood by 2.380 cases of pregnant women by nested PCR detection of Toxoplasma gondii in 4 cases,[link widoczny dla zalogowanych], the positive rate was 5.3 discussion immunosorbent assay IgM and IgG Toxoplasma gondii is a more sensitive and specific methods. Toxoplasma gondii IgG detection but may be subject to rheumatoid factor and antinuclear antibody in a false positive IgM} can also be affected by the impact of competition, false negative. When the immune suppression (such as AIDS) or the establishment of the immune system is not perfect (such as fetal, neonatal JL) ~ I restricted immune diagnostic rules. In 1989, Stanford University School of Medicine, who first of all to Toxoplasma gondii Bury multiple copies of B as the target genes to establish a PCR detection of Toxoplasma gondii. In 1992, we Maxim to the U.S. biotechnology company purchased the Burg primers (catalog number for a 1O03 TGO and TGO a 1004). Established by PCR amplification system,[link widoczny dla zalogowanych], found on the RH strain or internal amplification of DNA isolated from multiple bands appear,[link widoczny dla zalogowanych], shaped like a marker. After careful analysis of the Bt gene sequences and designed primers Burg, Burg set test results found in Table 1, Kunshan isolates nested PCR amplified products were sequenced and compared with the RH strain sequence shown in Figure 1. Wrapped in a strain of Toxoplasma gondii DNA prepared bearing in mind that children dichotoma tDNA-PCR showed downward pull mantis power in former times the same base meter uplink l upstream primer 3 'end of the 8 bases upstream and downstream primer 3' end of the six bases in the B repeated within the gene. To ensure the specificity and sensitivity of detection of Toxoplasma gondii, we have chosen a more than ordinary PCR sensitive and specific nested PCR techniques]. Specificity test showed that,[link widoczny dla zalogowanych], RH strain, and Xinjiang, Shanghai, Kunshan isolates were positive nested PCR results, and people and other organisms were negative results. Kunshan isolates nested PCR amplification products by DNA sequencing and Burg reported RH strain sequences. Sensitivity tests showed that nested PCR Toxoplasma sensitive than ordinary PcR 2 orders of magnitude. 80 cases of maternal peripheral blood by nested PCR detected four cases,[link widoczny dla zalogowanych], the positive rate of 5. Nested PCR to be used as two pairs of primers identified the second target gene, and a second amplification, the specificity and sensitivity are higher than a PCR (general PCR). Detection of Toxoplasma gondii by nested PcR the establishment of the diagnosis of human and animal toxoplasmosis molecular epidemiology of toxoplasma infection provide the means. 4