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scale fermentation broth into the tube,[link widoczny dla zalogowanych], 3000r/min centrifugal 10min. Supernatant, with the United States OI Chemical Company Flow Solution Ⅳ automatic analyzer in which the penicillin titer (u / ml). Precipitation volume and the portion size of the ratio of broth is the pmv (%).



2 results
ABSTRACT Using Penicillium chrysogenum # 87117 as starting strain,[link widoczny dla zalogowanych], a penicillin high yield strain # XY- 137 was obtained by UV irradiation combined with precursor-resistant strain in shake flask selection method under limited oxygen. Penicillin potency of strain # XY-137 was increased by 8.8% cultured in shaking flasks. Average productivity of about 100 batches fermentation level was increased by 2.69% ~ 5.66% than that of strain # 87117 in 100-ton fermentor.

1.3 strain mutation and selection

(3) resistant colonies detected in the precursor plate set 25 ℃ incubation 8d. Tolerance to different concentrations of precursors from treatment resistant colonies were selected to grow the spores are relatively abundant, the organization is relatively compact slant colonies were subcultured to prepare for shake-flask screening.




filter flask fermentation titer of the marker and mycelial growth (packed mycelial volume, pmv).
penicillin producing strain Penicillium chrysogenum 87117 #. 1.2 medium



1.4 sample testing
Key words UV mutagenesis; dynamic post-processing; rational screening; penicillin-producing strain
(3) fermentation medium with lactose, corn syrup as the main carbon and nitrogen sources, inorganic salts added component.
(1) separation and slant medium of sucrose, glycerol, yeast extract, NaCl, CaSO4, trace elements, agar.

2.1 XY-137 # high Mutants
the After penicillin raw materials processing and more extensive areas of semi-synthetic, highly competitive world market penicillin. Only manufacturers continue to increase production levels, reduce production costs to enhance the competitiveness of enterprises, and high producing strain and applications is one of the basic and key.
(2) seed culture medium by sucrose, corn syrup as the carbon and nitrogen source.
(1) the original strain by UV mutagenesis of single spore suspension is set at 40cm under UV light irradiation 90s.

Rational screening high-yielding strains of penicillin


Of: Lou Yu-Lian Zhang Xin Zhang Li Liu Jing Liu Jing Chen Lihua, He Jian-gong




strain 87117 # go through two natural selection,[link widoczny dla zalogowanych], and then combined with UV dynamic post-processing of precursor, 177 resistant strains were picked, the limiting oxygen conditions in the shake flask screening. Tolerance at 3.84% concentration of the precursor obtained XY-137 # high-yield mutant to 87,117 # for the control, the 5d and 7d were shaking the relative potency of 107.4% and 110.3%, shaking the average titer increased 8.8% .
1.1 starting strain

strains with penicillin production capacity, the limitations of traditional breeding methods become increasingly apparent. Screening is the application of rational principles of genetics and biochemistry, based on known or potential target product biosynthetic pathways and regulatory mechanisms of the molecular structure of the product, design method of screening, orientation and effective traits selected mutants. We based the concept of rational breeding,[link widoczny dla zalogowanych], with reference to the establishment of the basic screening conditions of the current production model, the vibration of industrial production strains UV stable genetic structure, supplemented by limited oxygen conditions, resistant mutant strains of precursors, to breeding high-yielding strains purposes.




KEY WORDS UV mutagenesis; Dynamic post-treatment; Rational screening; Penicillin high-producing strain
(4) directional filter flask oxygen consumption,[link widoczny dla zalogowanych], low-volume high-yield strains of mycelium will shake flask rotation speed reduced from the usual 220r/min 160r/min, under conditions of low ventilation flask screening. Characteristics of high-yield strains of the follow-up visits and process optimization are carried out under such conditions.
(2) the dynamic post-processing of precursor after absorbing ultraviolet irradiation quantitative spore solution were added with different doses of the precursor (acid amine) of seed culture medium in a test tube, so that each test tube final concentration of precursors in the 0 to 4.8%. Will set the 25 ℃ constant temperature oscillations in vitro cultivation of about 18h, then diluted in the solid medium plate separation.
1 Materials and methods
Abstract # to penicillin producing strain 87117 as the starting strain, the use of ultraviolet radiation complex dynamic post-processing of precursor methods, combined with limited oxygen flask under the conditions of selection, resistant strain of the body supporting the front from 177 to sieve out the XY-137 # high-yield mutant. Potency of the strain in shake flask an average of 8.8%, and the genetic characteristics of stability and strong product synthesis unit hyphae. Strain XY-137 # tank through the hundreds of tons of production validation batches, place it can titer, fermentation index, can grant an average of 3.83% yield, 5.66% and 2.69%. Chinese papers League finishing.

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