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Cholerny Spammer
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 Posted: Fri 9:45, 18 Mar 2011 Post subject: tory burch outlet Check the RhD blood type before |
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Check the RhD blood type before transfusion to ensure safety of blood transfusion on the clinical value of
Sun Xin Deng Jiye Division ● ■ -~-__-~ RhD saline method of safe blood transfusion in our department from September 1995 to carry out the Rh blood routine examination, the determination of Rh anti-D saline. 4 months 3167 cases from the results, the Rh blood type checks. Rh blood type of my regional population distribution, Rh type blood transfusion and safe blood transfusion with clinical significance and has practical value. Are as follows. 1 Materials and methods used by the U.S. Immcor company producing 1-1 Rh anti-D test Jing it applies to slide, rapid test tube method, micro plate method. Reagents and glass slide method 1.2 preheating. Add 1 drop of anti-D antibody reagent on the glass slide with a pipette} add 2 drops of 5 to 10, were seized on red blood cell suspension, shake the slide. Mixing, 2rain observe the results. Agglutination indicates the existence of cells in blood increases with the corresponding antigen reagent; not a lack of agglutination indicates that this antigen. 1.3 Quick test tube method was seized with saline red cell suspension was dubbed 2 to 5, with a pipette add 1 drop of red blood cell suspension was seized in a clean small test tube, add 1 drop of anti-D reagents,[link widoczny dla zalogowanych], mixing, 1000g centrifugation 15 ~ 20s,[link widoczny dla zalogowanych], gently shake the test tube. The red blood cell suspension,[link widoczny dla zalogowanych], observe the results. If the result was negative. Set the tube 37c incubated 15rain. Then centrifuged, observe the results. 2 results were found 3167 cases, 1646 cases of patients and relatives, RhD positive for 1640 cases, RhD negative 6 down, accounting for 0.36; blood donors, 1,521 cases, Rh-positive 1517 cases, RhD negative in 4 cases, accounting for 0.26. These results repeated with the Shanghai Blood Center, National Reference Laboratory of the RhD blood type test kit (with salt water method, respectively, papaya enzyme, anti-human ball reference test), the results were consistent. RhD negative results down to 10, with three batches of serum anti-D reagent (Shanghai Blood Group Reference Room) identified by 3 methods. The results were negative, to confirm the reliability of the reagent. 3 Discussion Rh antigen system includes more than 40 antigens. One of the most in the loop No. R446.62 Gen 7. Mainly the D antigen. Thick, usually based on the presence or absence of anti D to Rh negative and Rh system is divided into Rh positive. D antigen is the only attack on the A, B antigens of the most important antigen. Antigen negative patients receiving Rh D antibodies stimulate the production probability of very high D, C,, E, e antigen antibodies stimulate the corresponding probability of generating low; However, once the formation of D, C, c, E, e antibody, can mismatched red blood cell destruction. It shall be tested in the Rh blood type before transfusion. Often encountered in clinical blood transfusion and surgical patients repeatedly a large number of pediatric transfusion of blood and the smell of blood relatives. Screening and identification of Rh blood group D carried out, Rh blood type and blood group ABO blood typing simultaneously. 4 months found several cases of Rh negative blood, and several cases of patients with Rh negative blood type, blood transfusion safety has clinical significance. ABO blood transfusions at the same time to ensure to ensure the Rh blood type of blood transfusion was carried out with the purpose of this technology. If I need surgery, a surgical hospital. Preoperative blood type B, Rh negative blood type. Multi-Contact reserves the 1200mlRh by negative blood, so that surgery carried out smoothly. In another example,[link widoczny dla zalogowanych], children of a female surgical patients, blood type AB, Rh blood group of Lang, to be prepared blood 1800ml, be prepared before the surgery, the blood bank supply of the same type of blood,[link widoczny dla zalogowanych], made a successful heart surgery. If you can not lose the two patients with Rh negative blood type, will produce an immune response. Rh blood group carried out the survey so the change of a single type of blood transfusion with ABO blood groups, ABO blood type and Rh blood type to the same type of blood transfusion. This is to ensure safety of blood transfusion in our hospital a viable measure. RhD blood group to carry out checks on my understanding of regional distribution of Rh blood type and Rh-positive individual friends or Rh blood shaped infusion can produce a large number of antibodies needed to do careful clinical observation. Rh blood group of the research, the conduct of the examination should be given adequate attention. Experimental results show that: in ensuring safe blood transfusion has a practical value.
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