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yoylqnqvwb · Posted: Thu 20:26, 17 Mar 2011 Post subject: tory burch agz ica gxl jde

,[link widoczny dla zalogowanych]
Ganoderma lucidum spore oil on N- methyl -N- nitrous urea induced retinal injury in the expression of Caspase-3

emaleSprague-Dawleyrats (50 a day-old) weredividedinto4groupsrandomly,[link widoczny dla zalogowanych], includingnormalcon-trolgroup (NC),[link widoczny dla zalogowanych], untreatedMNUmodelcontrolgroup (MNU),[link widoczny dla zalogowanych], docosahexaenoicacidtreatmentgroup (DHA) andganodermasporeoiltreatmentgroup (GSO). Retinatissuesampleswereobtainedsequen-tially0hourbeforeMNUinjectionand1, 3,7,10 daysafterMNUinjection. ExpressionsofCaspase a 3weredetectedbyRT - PCRandimmunofluorescenceas ·-says,[link widoczny dla zalogowanych], thenphotoreceptorcellapoptosiswasconfirmedbyterminaldeoxynucle0tidyltransferase-mediatedde-oxyuridinetriphosphate ·-digoxigeninnick · · endlabeling (TUNEL) signals. Results ① Caspase-3mRNAex-pressioninMNUgroupup-regulatedonldandpeakedon3dcomparedwithNC (P <0.01). TheexpressionlevelsofDHAandGSOgroupdecreasedobviouslyon1d, 3d (P <0.01usMNUgroup), furthermore, thatofGSOgroupon1dwaslowerthanthatofDHAgroup (P <0.01). ② ImmunofluorescenceassaysshowedthatCaspase a 3expressionofDHAandGSOgroupinretinadecreasedclearlyon3dcomparedwithMNUgroup. Moreover, GSOdown-regulatedCaspase a 3ex-pressioninretinaalittlebitmorethanDHAdidon3d. ③ Photoreceptorcellapoptosiswasonlyevokedintheouternuclearlayerandpeakedon3dinMNUad-ministeredrats. Theapoptoticindexes (AI) ofGSOgroupandDHAgroupwerelowerthanthoseofMNUgroupon1d, 3d, respectively (P <0.01). ComparedwiththatofDHAgroup, AIofGSOgroupwasreducedsignificantlyonlyon3d (P <0.01). ConclusionGSOmayinhibitMNU-inducedratphotoreceptorcellapoptosisviadown - regulationofCaspases-3expres - sion. Keywords: N-methyl-N-nitrosourea; photoreceptorcell; apoptosis; ganodermasporeoil; d0c0sahexaen0icacid