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Cholerny Spammer
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 Posted: Mon 15:23, 14 Mar 2011 Post subject: timberland scarpe Influence of lanthanum on the ro |
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Influence of lanthanum on the role of root surface caries resistance of the minimum effective concentration
onclusion: ThereiSacloserelationshipbetweenpmliferationanddifferentiationofskeleta1musclecellsinvitro. proliferationofmyoblastsisthepreparedfordifferentiationforfuture. whendifferentiationbegins, cellshavetoexitcellcycle,[link widoczny dla zalogowanych], thenumberofcellsdecline, andremainsteadyaftertheformationofmyotube. [Keywords] skeletalmuscle; myocytedifferentiation; proliferation generally considered to have mature muscle does not have regenerative capacity , since the muscle satellite cells found , along with the constant depth of its research , people gradually realize that the satellite cells to muscle cells to regenerate damaged Potential. Mauro , who found that muscle satellite cells can be activated in the injury , differentiate into myotubes fused with each other ,[link widoczny dla zalogowanych], participate in muscle repair l1.2]. Visible muscle cell differentiation and regeneration of mature muscle cells for an extremely important role in l_3J,[link widoczny dla zalogowanych], in the denervated muscle damage and the treatment of muscle atrophy has important practical significance. The study is to preliminary study of the proliferation and differentiation of skeletal muscle cell relationship. Materials and Methods 1. Materials C2C12 cells (Proton kindly Professor ),[link widoczny dla zalogowanych], DMEM medium (Gibco, USA),[link widoczny dla zalogowanych], fetal calf serum (Gibeo, USA), tetrazolium blue (M1vr) (Sigma, USA), 450 microplate reader (Biorad Company). 2. 2.1 Cell culture methods take C2C12 cells , 2.5g / L trypsin digestion, wind and percussion straw , made of cell suspension , adjusting the cell concentration 1X104/ml, seeded in 96 well plates , each well 200ul, respectively, containing 100ml / L or 2Oml / L fetal calf serum in the DMEM culture, then home culture incubator (5% CO2, 37 ℃), to log [
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