lin91957
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Posted: Wed 9:36, 02 Mar 2011 Post subject: RP—HPLC测定牡 |
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RP—HPLC测定牡丹皮饮片中3种活性成分的含量
hromatogr,2004,18(10):785.杨更亮,宋秀荣,张红医,等,胶束毛细管电泳法测定牡丹皮及芍药中牡丹酚和芍药苷[J]分析化学,1999,27(1):1.赵新峰,孙毓庆.胶束电动毛细管色谱法测定牡丹皮及六味地黄丸中丹皮酚的含量[J].沈阳药科大学学报,2002,19(1):35.丁安伟,郭戎,徐菊如,等.丹皮炭中丹皮酚含量的高效液相色谱测定[J].中国中药杂志,1996,21(1):23.葛萍,雷岁合,曹新宪.HPLC法测定益智壮骨冲剂中芍药苷的含量[J].中成药,1996,18(2):13.SimultaneousdeterminationofthreeactiveconstituentsinCortexMoutanbyRP.HPLCWANGYu—wen.YUKe.CHENGYi—yu(DepartmentofChineseMedicineScienceandEngineering,CollegeofPharmaceuticalSciencesZhejiangUniversity,Hangzhou310027,China)[Abstract]0bjective:ToestablishaRP—HPLCmethodforthesimultaneousdeterminationofgallicacid,paeoniflorinandpae—onolinCortexMoutan.Method:TheanalysiswascarriedoutonacolumnofYMC—Cl8(4.6mm×250mm,5m),andgradientelu—tionwasusedforthebaselineseparationofthesethreeconstituentswiththedifferentdetectionwavelength.Result:Thelinearrangesforgallicacid,paeoniflorinandpaeonolwere0.0130-0.267,0.0880-1.76and0.164—3.28g,respectively.Theiraveragerecover—ieswere104.3%,101.7%and99.5%,respectively.Conclusion:Themethodwassuccessfullyappliedtothedeterminationofgallicacid,paeoniflorinandpaeonolinCortexMoutanfromthedifferentdrugstores.Itwasshowninthisstudythattheproposedmethodcouldbeusedtoevaluatethequalityofthemedicinalplantsorherbalpreparationscontaininggallicacid,paeoniflorinandpaeono1.[Keywords]RP—HPLC;multipledetectionwavelengths;CortexMoutan;quantitativeanalysis[责任编辑李禾]本刊投稿须知本刊目前一律不收网上投稿,请作者务必以邮寄方式投稿。来稿应为打印稿,一式两份;投稿时不必附软盘;实验性文章应附单位介绍信。投稿同时请从邮局汇寄处理费(50元/篇),切勿在稿件中夹带现金;汇款时请注明文章第一作者姓名及稿件题目。本刊将于收到稿件后的1个月之内给予回执及发票。·l701·
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