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2.2 Panax size study hard texture, particle size on extraction rate of the drug to a certain extent. Therefore, the total saponin extraction rate index, its size and examine the relationship between the yield.



2.2.1 Preparation of the test solution were weighed Panax most meals, meal, all in powder 30 g, accurately weighed, add 70% volume fraction of 7 times the amount of ethanol, extracting 2 times, each time 1.5 h, filtration, combined filtrate, respectively, to volume 500 mL, as the sample solution. Precision Pipette 2 mL of the sample solution evaporated water bath, allows the dissolved residue in methanol, set the volume to 10 mL volumetric flask, as the test solution.

Table 1 on the extraction rate of particle size (a little)

Figure 1 notoginseng by high performance liquid chromatogram (omitted)
2 methods and results Panax notoginseng contained in R1, R2, ginsenoside Rb1, Rd, Rg1 and other saponins (hereinafter referred to as saponins, PNS) for the main active ingredients [2], such components soluble in methanol, ethanol and water. The pre-test, with water as solvent, a total of Panax fried with prescription drug group, the total transfer rate of only 30% saponins, therefore, to determine notoginseng extract alone. And the volume fraction of water were used 70% ethanol as solvent extraction, solvent extraction of water for the ethanol extract of the total amount of saponin is 52.6%. Preliminary pharmacodynamic experiments show that ethanol extract of Panax notoginseng extracts and group syrup mixture, ulcerative colitis in experimental animals improved significantly the role of [3], it is OK to ethanol as solvent, the use of orthogonal test , optimal extraction conditions Panax.
Key words: Radix notoginseng; total saponins; compound Huangqi Changning enema; orthogonal design; extraction technology


A. B. reference standard samples
2.2.2 Determination of precision drawing reference solution, respectively, and the test solution 15 μL, into the liquid chromatograph, press the saponins Rb1, Notoginsenoside R1 peak area and calculate the results in Table 1.
Abstract: Objective To optimize the extraction procedure of total saponins from Radix notoginseng for compound Huangqi Changning enemA.Methods Orthogonal test was employed for optimizing the extraction processes by the indexes of the contents of ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1.Results Six volumes of 70% alcohol, 3 times of extraction processes with 1.0 hour for each time were considered as the optimum extraction technology. ConclusionThis extraction procedure of active components in the drugs is achieved by applying the selected technology.





Compound Huangqi Changning agents from Astragalus, Panax, psoralen, Agrimony etc., with kidney and spleen, dampness heat, pass through the myogenic effect, for the treatment of ulcerative colitis. Clinical use of decoction enema administration, enema Panax liquid to powder added. But the fine particles produced stimulation of the intestinal mucosa, causing a sense of bowel movements, resulting in intestinal enema retention time shortened, reducing efficacy. In this paper, orthogonal experimental design to extract Ginsenoside Rg1, Ginsenoside Rb1, Notoginsenoside R1 content as an indicator of optimal extraction conditions notoginseng for production of even more convenient, provide the basis for effective preparation.

2.1.1 column chromatography conditions Kromasil C18 (250 mm × 4.6 mm, 5 μm); mobile phase: acetonitrile mobile phase A, with water as the mobile phase B, gradient elution: 0 ~ 90 min, mobile phase A from 19% to 36% mobile phase B from 81% to 64%; flow rate: 1 mL · min-1; injection volume: 15 μL; column temperature: 28 ℃; detection wavelength: 203 nm. Peak notoginsenoside R1, ginsenoside Rg1 peak of ginsenoside Rb1 peaks, respectively, 23.18 min, 28.54 min, 83.90 min. Figure 1.
2.1 Determination of PNS [1]


results show that the coarse powder higher extraction rate of PNS,[link widoczny dla zalogowanych], it is to determine particle size meal notoginseng.
Key words Panax; PNS; Compound Huangqi Changning agent; orthogonal test; extraction process



1 instrument and reagent performance liquid chromatography (WATERS2695/2996). As Araliaceae Panax Panax notoginseng (Burk) FHChen dry roots and rhizomes, were purchased from Guangzhou,[link widoczny dla zalogowanych], sent a letter to Chinese Herbal Medicine Co., Ltd., through the determination of [1], in line with a) thirty-seven under the provisions of ginsenoside Rb1, ginsenoside Rg1, notoginsenoside R1 and for the three mass fraction of 8.86%. Ginsenoside Rb1 (110704-200420), ginsenoside Rg1 (110703-200424), Notoginsenoside R1 (110745-200415) Pharmaceutical and Biological Products in China are provided; methanol, acetonitrile for chromatography pure; other reagents were of analytical grade.
【Abstract】 Objective To optimize the compound Huangqi Changning agents the best extraction Panax. Orthogonal experimental design method,[link widoczny dla zalogowanych], using high performance liquid chromatography ginsenoside extract Rg1, Ginsenoside Rb1,[link widoczny dla zalogowanych], Notoginsenoside R1 content, preferably the extraction process notoginseng. Results of optimal technology for 70% ethanol 6 times, extracting 3 times, each time 1.0 h. Conclusion The optimal extraction rate of PNS extraction for production. Chinese papers League finishing.
Compound Huangqi Changning agent Optimum extraction process of Panax


Of: Zhang Chao Lin, Li Yong, had fertility Jun, Chen Xuemei

Fig.1 HPLC chromatograms of Radix notoginseng 2.1.2 standard curve, accurately weighed Ginsenoside Rg1, Ginsenoside Rb1, R1 notoginsenoside appropriate reference substance,[link widoczny dla zalogowanych], add methanol produced per 1 mL containing ginsenoside Rg1 1.071 mg, ginsenoside Rb1 0.994 mg, notoginsenoside R1 0.518 mg of the mixed solution, as the reference solution. Precision drawing reference solution, respectively, 3,5,10,20,25,30 μL, into the liquid chromatograph, chromatographic conditions were measured by the peak area to ginsenoside Rg1, Ginsenoside Rb1, R1 mcg notoginseng number of cross- coordinates (X), peak area value of the vertical axis (Y), calculate the regression equation. Ginsenoside Rg1: Y = 274278.66Y-81840.47 (r = 0.9999), linear range of 1.285 ~ 12.852 μg; ginsenoside Rb1: Y = 216171.23X-125333.54 (r = 0.9997), linear range of 1.193 ~ 11.928 μg; Panax saponin R1: Y = 239798.93X-38810.58 (r = 0.9999), linear range of 0.622 ~ 6.216 μg.

Tab.1 Effects of particle size on extraction efficacy More articles related to topics：


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