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Cholerny Spammer
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 Posted: Mon 21:13, 17 Jan 2011 Post subject: polo hoodie In vitro refolding of recombinant prot |
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In vitro refolding of recombinant protein
roteinlye-foldinginrevel'semicell ~. BiotechBioe ~ g, 1990,35:955 ~ 96514Garza-RamosG. TuL = ~ flaGP, GonlPl-PuyouA, etaI_Dimedzadonandreactivationoftriosephm-phate ~ orneraseinrevelt ~ emlcdlesEur】 Biochem, 1992,[link widoczny dla zalogowanych],208:389 ~ 395.15Kabanov. KlyachkoNL, NametkinSN, etalEngineenngoffunctionalsupramacromolecolarcomplex ~ ofproteinusingrev ~ gg4 * dmicelles8smaedxmJcToreactorsProteinEngineer, 1991.4:1009 ~ L017.16ChelandJL, BuilderSE, SwarrzJR. etalPolyethyleneglycolenhancedproteinrefoldingBio / Tech, 1992,[link widoczny dla zalogowanych],10:1013 ~ 10l9l7CD Ⅲ nsFD, LileJ, BekteshS. etalProductionofbiologicallyactive, recombinantmemberoftheNGF / BDNFfamilyofneurotrophicproteins. Eu-Fope ~ TtPatent, C12N ,0450386.1991-03-1818C ~ sollJD. YarmushML_Antibodyassistedpro-reinrefoldingBb/Tech, 1992,10:86 ~ 9119TamJP. WucR, LiuW, etaI. Dulfidebondformationinpeptide ~ bydimethylsulfco ~ ide. JAmChemSoc, 1991,113:6657 ~ 666220CedettiN. NovelprocessfortheproductionofbiologlcallyactivedimefieproteinWopatent. C07K. 96034331996-02-08. Cell Aging Secret Wemer's syndrome to study the genetic mechanism, molecular biologists Guarente and Davidsmchir using yeast Saceharomycescerev / siae research. This is because the Wei-llers is characterized by premature aging, and yeast Sacchammycesce ~ evisiae genes relating to aging of the SGS1 gene sequences with the same period of the human body. The yeast is usually a period of 25 generations of sexual reproduction. If the SGS1 gene mutation, then it dropped to 9 generations of sexual reproduction and the state was premature. From the morphological point of view. When SGSI gene mutation, the yeast cells than normal loose edge kernel. Moreover,[link widoczny dla zalogowanych], using a special marker to observe, that the yeast cells before breeding. Mother cells, DNA replication at the same time, there is a small amount of rDNA during replication. The rDNA copy only in parent cells,[link widoczny dla zalogowanych], when this copy to a certain number of rDNA,[link widoczny dla zalogowanych], which is equivalent to yeast cells when the total DNA content. Nucleus began to crack, cell aging. If the SGS1 mutant cells. RDNA copy of its intracellular faster rate than normal cells. The mutant cells easy to aging SGSI. If the copy of this rDNA add another line of yeast cells, can make another line Shao short life span of yeast cells in 42%; if trying to stop cells in rDNA replication. The cell life span will be extended by 25%. The results on the display, cellular senescence is controlled by a specific number of rDNA replication, with a precise time control mechanism. Is this common in mammalian cells, it is worth further study. (Science, 1998,279:34) (Zhu Xiaosong Li Shao),,,
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