Forum pracy nad projektem wskrzeszenia The Forge

[yoylqnqvwb]

Spiramycin biosynthesis traffic analysis


l-butionsmPe. icillium,[link widoczny dla zalogowanych], gnl Biotechno [Bioeng,[link widoczny dla zalogowanych], 1995; 46:117 [8] Foi] stadBD-I ~ ttcarceiRR,[link widoczny dla zalogowanych], StephaG. MetabolicfluxanalysisofhybridomaCOIllitpdOU ~ cu [turesteadySilttenlul-tip] icity [J]. Biotec] mo [Bioeng, 1999; 63 (6): 675 [9] OmuraS. Bhonversionandb [osynthesisot16-memberedmacrolMem ~ 6biotics. XFinalstepsintilebiosynthesisofsp [ramycin. usingenzymeinbibior {ceru] enin mouth ]. CbenlPharillBuII, 1979; 27 (1): 176 [10] Lee Yuan Chen Changhua . THEORETICAL AND , et al. Kinase and acyl CoA acyl enzyme units into units rotating into the mold of cable [J]. East China University of Technology, 2001; 37 (3) 251 [1 】 ] KitaoC. O,[link widoczny dla zalogowanych], uuraS. Bioco, iver. g ~ ollandbiosynthesisotl6 a memberedmacrolideandbioties. Xl: Regulationotsplramycin 【 3-hydroxylac] aseformationbyg] ucosebmyratealldceru [eniaD]. JAudb [ot, 1979; (6): 593MetabolicfluxanalysisofspiramycinbiosynthesisLiYou - yuanandChenChang - hua (StateKeyLaboratoryofBioreactorEtlglneerlug.ECUST, Shangllai300237) ABSTRACTByanalyzingtheorganicacidsandotherintermediarymetabolicproductsduringspiramyc [nbiosynthesis, thedynamicfluxdistributionprofileswereestablishedBasedontbesefindings, theknotpointsofthemetabolicnetworksofthebioconversionofspiramyclnwereinvestigated. Resuhsrevealedthatthe14-memberringformingprocessandthetransformfromF0 - 1tONSP - 1werecriticalduringthefermentationprophase. TheefficiencyofspiramycinproductionwascloselyrelatedtothereductionOfFO-2andNSP-2whichwereaccmulatedsimultaneouslyduringringformationItwasconformedintheflaskexperiments, theadditionofglucoseproducedapositiveeffect0nthebiosyn-thesisofspiramycm,[link widoczny dla zalogowanych], withanincreasedpotencyby26.8. KEYWORDSSp [ramycin; Biosynthesis {Metabolicfluxanalysis