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Polymerase chain reaction identification of sex and cow embryos to transplant


ulDH52, agar plate containing Amp training, select the white seedlings down. Then add 50ul recombinant paraffin oil covering the body. And then Southern hybridization and dot hybridization screen and then 05 ℃ 1 分钟, 5s ℃ 1 分钟, 72c1 minutes. Hand selected, select it with the Bulls of hybridization with the specific DNA clones, the sequence line 3O 20 cycles of amplification products by agarose gel electrophoresis with a check, if the price of cottonseed cake enhanced detoxification 1l0 / dry g, feed with Taiwan the proportion of testis in bread by 20 cotton count, per hundred can save l6 per kg of feed, then feed consumption ratio of 1:2.68, the average count. Eggs can be saved per kg more than 0.43 per benefit. 3. Enhanced microbial detoxification of gossypol content of cottonseed cake not only significantly decreased (the original 82fippm), protein content increased (the raw materials we use are trapped II cotton seed cake protein content so that only the original 321),[link widoczny dla zalogowanych], and egg acid, lysine acid, fine acid were also added to the original 1.31,3.86,10.2 1. . 3.97,11.87, but also increased B vitamins, is currently working to further improve the protein. Exchange of experience is Y chromosome specific fragment of 33 DNA amplification with the females. Results for the male sample. Otherwise, the table shows. Calves born seven PCR identification of gender and sex of embryos was consistent】 known sex cow blood products collected 6 deleted wife seized cows,[link widoczny dla zalogowanych], four-dimensional samples of blood of the bull, crude WBC was conducted after the sample treatment . PCR amplification and then add the ingredients, the results shown in Table 1. Decline a blood sample of known sex cow test results on samples seized 123456789l0PCR No. male and female parents-resistant parent parent parent parent parent parent parent phenotype of the male and female sex female parent, mother father 2. Fetal gender test results will be six cow embryos into 3 parts, 2 cut into 2 copies. Each cell number by a few to dozens of varied treatment by the sample. For PCR amplification. The same mix products of different embryonic sex determination results in Table 2. Decline of the same embryo 2 2 salt, were cut after the test results do 3. Identification test after transplantation of fresh embryos collected in the dairy, cutting out dozens of cells, using PCR methods that can identify gender after transplantation. 7 calves born. The results in Table 3. Decline of 3 cow embryos depleted by the sex identification of trespassing after a discussion of the results calving established the specificity of PCR detection using PCR primers designed. The cow blood samples and fetal cells Wang Y chromosome DNA amplification,[link widoczny dla zalogowanych], from Table 1, Table 2 and Table 3 shows. Amplification of bovine blood samples of known sex and the same embryos result in full compliance with different samples of cow embryo sex reason I check the results of j-born seven calves with gender results were in agreement. Established in this study showed that PCR amplification of Y chromosome fragments were specific fragment. 2PCR reason I check the sensitivity j Because PCR has high sensitivity, vulnerability to pollution during the operation. Caused by non-specific amplification,[link widoczny dla zalogowanych], and reduce the detection accuracy, especially PCR product easy to form a Therefore, the operation must be in strict accordance with the operating specifications for PCR to avoid non-specific amplification. High sensitivity of PCR technology. A molecular target can be amplified sequence has been amplified in the experiment we had a cell. Thus, for sex identification number of embryonic cells in the sample can be just a few cells. Such as 2 ~ 5 cells, but because of the difficulties of cutting samples, difficult to get into such a small number of cells in the sample if the saddle to take less. The number of cycles required for amplification may be increased. 3. Mixing technology to take embryos according to China's actual situation. We have established a set of fetal sampling techniques. Using hand-held glass needle, cutting embryo cells in the dissecting microscope sample; increase the bottom friction plastic plate to fix the embryos} draw embryo samples, do not inhale the zona pellucida. Which may carry the death to prevent sperm contamination. So as to achieve high-quality embryos for the purpose of 4 samples. For the application of PCR in the actual production of embryonic sex identification,[link widoczny dla zalogowanych], we have the embryonic sex identification of the PCR kit preparation, preliminary results have been obtained, embryo sex determination by cutting after sampling and kept frozen Ling t-test once they have been test access, application of PCR technology will enable sex identification methods Wang practical cabin and supporting technology, embryo sexing technology will further the application in production, so that high-tech as soon as possible into productive forces.
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