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Eucommia extract on estrogen deficiency mice induced by


Of: Fan Jing Lu Gui Li Wei Zhang Yixun source Liheng Hui Zhang Su Jie Chen Su-Hong
2.2 Monitored general signs in mice. Administration of drug administration for the first 3 days and 12 days measured rectal temperature. 3 days before administration of the first 13 days delivery time for determination of swimming, more than 1 hour to 1 hour. 2 days before administration of the first 14 days of administration determined grip, continuous determination of each mouse 2 times the average. 1 day before the first dose administered 15 to 16 days, recorded 10min locomotor activity in mice. After the last administration, blood eyeballs, one without anticoagulant, serum taken, according to the instructions of serum Cr, UR; another plus sodium citrate, blood blood indices measured after 4 hours : red blood cell count (RBC), hemoglobin concentration (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet count (PLT), white blood cell count (WBC), addicted to the the number of neutrophils (NE), lymphocytes (LY), the number of monocytes (MO), the number of eosinophils (EO), the number of basophils (BA). Mice were killed, the removal of testes, seminal vesicles, calculated index (%) = organ weight / body weight × 100%.

3.1 on the general signs of the control mice fur dry smooth, good spirits, hi, hi, lying without moving. Model mice fur sweat erected, apathetic, arched atrophy, rather than lying hi hi squeeze move. Eucommia extract early in the experiment in each group (administration of 1 to 7 days) also appears with the model group were the same signs, but in the experimental period (8 to 15 days administration) gradually returned to normal.
1 experimental material
3.2 Influence of perianal temperature before administration in each group no significant difference between the rectal temperature. Compared with the control group, administration of the first 12 days, the model group was significantly lower rectal temperature (P <0.01), successful model that the temperature drops; with model group, Eucommia water extract group and the ethyl acetate extract group was significantly rectal temperature increased (P <0.05), n-butanol extract of the rectal temperature tended to increase. Results suggest that deficiency in mice Eucommia has increased the role of rectal temperature in Table 1. Table 1 Comparison of rectal temperature of mice compared with control group,
3.3 hours of swimming in each group before administration was no significant difference between the swimming time. Compared with the control group, administration of the first 13 days, the model group significantly reduced swimming time (P <0.01), that successful model Yaoxisuanruan; with model group, Guifu Huang Wan Group, Eucommia water extract group and n-butanol extraction group was significantly longer swimming time (P <0.05). Results suggest that there may be improvement Yaoxisuanruan Eucommia role, see Table 2.
Key words Mouse Kidney
1.1 Eucommia drug salt, lot number 080110, was purchased from Zhejiang Medical University medicine plant, plants identified as Eucommia Eucommia Eucommiaulmoides Oliv. Bark of the processed products . Water extract of the first made of herbs; then take the medicine with 10 times the volume of 95% ethanol extract 2 times, respectively, made of ethyl acetate extract, n-butanol extract. Water, butanol, ethyl acetate extract were added to 0.125g of Tween 80 diluted crude drug / mL.
1.3 Animals ICR mice, male, weighing 18 ~ 22g, by the Experimental Animal Center of Zhejiang Province, Certificate of Conformity: SCXK (Zhejiang) 20030001.

2.1 modeling and administration of health 72 ICR male mice were randomly divided into control group, model group,[link widoczny dla zalogowanych], Guifu Huang Wan Group, Eucommia water extract group, butyl alcohol extract group, ethyl acetate extract group, a total of 6 groups of 12. In addition to the control group, the other 5 groups according to body weight by intraperitoneal injection 0.4mg/mL 0.1mL/10g estradiol benzoate soybean oil diluent, 1 day 1 for 15 days, resulting in deficiency model [1]. Eucommia extract 0.125g each group to give the corresponding crude drug / mL extract, to give 0.15g crude drug Guifu Group / mL suspension Guifu Huang Wan, control group and model group were given 0.4% Tween 80,0.1 mL/10g ig, 1 day 1 for 15 days.
1.2 Reagent creatinine, urea kit (Cr, UR, Shanghai Changzheng Medical Science Co., Ltd. Revival, batch number P071254, P080431). Blood kits (U.S. A Drew Scientific Group Co., Batch number 5018). Estradiol benzoate (Shanghai GM Pharmaceutical Co., Ltd., batch number 070902). Guifu Huang Wan (batch number, Lanzhou too Bao Pharmaceutical Co., Ltd., 80,080,542).
1.4 instrument HEMAVET950 animal blood analyzer (U.S. A Drew Scientific Group Co.); XZ4 5-Kwong Road, spontaneous activity logger, YSL 13A and mice grip tester (Shandong Academy of Medical Sciences equipment supply service stations); Rittal SELECTRAE automatic biochemical analyzer (He Lanwei Chart Company); DTITB electronic thermometer (Shanghai Hua Chen Medical Instruments Co., Ltd.); LP123 electronic balance (scale plant in Changshu City, Jiangsu Province).
China, \fetal movement instability. \Eucommia can be used for treatment of kidney deficiency caused by the card. To explore the nature, taste, owned by the content of modern science,[link widoczny dla zalogowanych], to carry out the following studies.
3.4 对 of grip between the groups before administration no significant difference in grip. Compared with the control group, administration of the first 14 days, the model group mice were significantly reduced grip (P Table 3 Comparison of the grip of mice
Abstract Objective: To observe the effects of extracts from Cortex Eucommiae on estradiolinduced kidneyyang deficiency in mice and to understand the modern scientific connotation of the properties theories about sweetflavor, warm nature, meridian tropism in liver and kidney. Methods: Kidneyyang deficiency mice model was established with intraperitoneal injection of estradiol benzoate. A total of 72 healthy adult male ICR mice were randomly divided into 6 groups: control group, model group, positive control group (Guifudihuang), and 3 groups of Cortex Eucommiae (water extract, nbutanol extract, and acetoacetate extract), 12 mice in each group. The effects were observed by measuring physical sign, rectal temperature, grip strength, swimming time, locomotor activity, serum creatinine and urea levels, haematological indexed, testis index and seminal vesicle index. Results: The water extract markedly improved the rectal temperature, swimming time, locomotor activity, testis index, seminal vesicle index, Hb, WBC, LY, MO and PLT, and decreased urea. The nbutanol extract had the similar effects as the water one did except for increasing RBC instead of the rectal temperature. The acetoacetate extract dramatically improved the rectal temperature, grip strength,[link widoczny dla zalogowanych], seminal vesicle index, Hb and PLT, and decreased urea and creatinine. Conclusion: These effects of Cortex Eucommiae could be as the basic of improving the symptoms of kidneyyang deficiency such as soreness of the waist and knees, hyposexuality, intolerance of cold and cold limbs, feeling listless and inert, edema, and blood deficiency that is induced by kidneyessence and liverqi deficiency, and also could be an important part of the modern scientific connotation of the properties theories about sweetflavor, warmnature, meridian tropism in liver and kidney. The effects of the water and nbutanol extracts were similar to each other and both stronger compared to the acetoacetate one.
Effects of Cortex Eucommiae Extracts on Estradiolinducecd Kidneyyang Deficiency Mice FAN Jing, et al. Zhejiang Chinese Medical University, Hangzhou (310053),[link widoczny dla zalogowanych], China
Abstract Objective: To Eucommia extract on estradiol deficiency in mice. Understanding of Eucommia \Methods: Intraperitoneal injection of estradiol benzoate deficiency model in mice. 72 ICR male mice were randomly divided into control group, model group, Guifu Huang Wan Group, Eucommia water extract group, n-butanol extract group, ethyl acetate extract group, a total of 6 groups, n = 12 . Signs of mice, rectal temperature, grip, swimming time, locomotor activity, serum creatinine (Cr) and urea (UR), hematologic indicators, testicular and seminal vesicle index were determined. Results: The aqueous extract of Eucommia deficiency in mice can increase the rectal temperature, swimming time, autonomic activity, testicular and seminal vesicle index, Hb, WBC, LY, MO, PLT, reduce the UR; butanol extracts of mice grip, swimming time, locomotor activity, testicular and seminal vesicle index, RBC, Hb, WBC, LY, MO, PLT, reduce the UR; ethyl acetate extract could improve the mouse rectal temperature, grip, seminal vesicles index Hb, PLT, reducing Cr, UR. Conclusion: the pharmacological effects of Eucommia can be used to improve the deficiency syndrome of the waist and knees, loss of libido, aversion to cold, apathetic, Yang and water pan, and Kidney essence deficiency,[link widoczny dla zalogowanych], qi deficiency of blood due to weak foundation; and as its \Eucommia water extract and butanol extract on closer and stronger.
2 experiments
2.3 (± s) that was used to compare groups ttest test.
Statistical analysis of data to
Key words mice Cortex Eucommiae kidneyyang deficiency extract property and flavor meridian tropism
Eucommia extract character, taste
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