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Cholerny Spammer
Joined: 17 Nov 2010
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Location: England
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 Posted: Thu 5:17, 17 Mar 2011 Post subject: MBT Cipő HMGA1 siRNA on gene expression in epithel |
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HMGA1 siRNA on gene expression in ovarian cancer cells suppress experimental study
plorethesignificanceandapproachofinducingcultureandcryopreservationinhumanperipheralblooddendriticcells. Methods: Wefondthebetterexperimentalprojectofmaturingandinducingthein-creaseofdendriticcellsbyisolatingandculturing, processingandloadingantigen,[link widoczny dla zalogowanych], classingcryopreservingcells, eta1. Results: Dendriticcellsderivedfromperipherialbloodmonocyte, aftertheywerethawedandobtainedbioactivityaddingGM-CSF, IL-4. Condusion: Thereissignificantimplicationininducingcultureandcryopreservationinhumanperipheralblooddendriticcells. 【Keywords】 dendriticceHs; inducingculture; CryopreservationModernOncology2008, 16 (7) :1082-1084 Abstract Objective: To investigate peripheral blood dendritic cells (Dc) of the induction culture and cryopreservation methods and significance. Methods: dendritic cell isolation and culture, antigen pre-treatment samples, antigen loading, experimental group, cryopreservation, etc.,[link widoczny dla zalogowanych], and explore the expansion induced by dendritic cells and promote maturation of the optimal experimental scheme. Results: Peripheral blood mononuclear cells can be used as a source of Dc, GM-CSF and IL ~ 4 can induce the differentiation of precursor cells to the Dc, added cytokines can promote the mature cells to maintain cell biological activity. Conclusion: The induction of human peripheral blood cells Dc culture and cryopreservation of great significance. Dc frozen cells maintain its properties, but because of some biological characteristics of the process of reducing the freezing and thawing, Dc cells frozen in the application of this should be considered to increase the amount of input cells. . Key words dendritic cells; induction; frozen 【Key Words】 R730 Document code】 【A】 【Article ID 1672-4992 a (2008) 07-1082-03 dendritic cells (Dendriticcells, DC) is the body's most powerful professional antigen presenting cells that can efficiently uptake, processing and presenting antigens. In recent years,[link widoczny dla zalogowanych], as the immune cells to carry out the treatment Dc cancer, hepatitis B and other studies are very active, the cells in order to more effectively applied to the clinical purpose of this study on dendritic cells in vitro amplification test methods assessment, and promoting Received Revised Fund of units of Informal 2007-07-2120o7-11-12 Research Foundation of Zhongshan City,[link widoczny dla zalogowanych], Guangdong Province (Grant No.: 2005A137) Cancer Institute, Zhongshan Hospital, Zhongshan University, Guangdong Zhongshan 528403 Fanghui Yun (1972 a), female, Hubei Ezhou, Master, is mainly engaged in the work of Cancer. Cells were identified by mature cells, of a expansion induced by dendritic cells and promote maturation of the optimal experimental scheme. Specific reports are as follows: 1 Materials and methods 1.1 Isolation of dendritic cells by conventional methods and culture ① mononuclear cells isolated from washed cells with PBS 2 times. (1300rpm × 10 minutes). ② 10% FCSRPMI1640 training 3 hours, (25cm flask placed 1 × 10. Cells), DC to try to provide the principle of surface adhesion. ③ Rinse: A mild intensity of shaking flask, the adhesion of T cells are not washed, drawing liquid and not adherent cells. (Note: try to wash away the T cells retain the adhesion of DC cells). ④ back into the new medium, repeat ③ action again. ⑤ add a new medium 5ml / culture flask,[link widoczny dla zalogowanych], adding the medium per milliliter 1000UGM-CSF, 500UIL a 4; ⑥ 5% CO237 ℃ to continue to foster, 2
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