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Cholerny Spammer
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 Posted: Mon 5:28, 31 Jan 2011 Post subject: ghd nederland Organotin Analysis Methods _184 |
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Organotin Analysis Methods
bergE, eta1. [J]. FreseniusJAnalChem. 200o ,366:400-407. WahlenR, BricheCW. [J]. AlaBioanalChem. 2003,[link widoczny dla zalogowanych],377:140-146. Peter turns to write r. 1q and worship of a 1. International Journal of Health Toxicology 2005, 32,[link widoczny dla zalogowanych], No. 5 305 · [30] WuJC, eta1. .... AnalAtSpectrom, 2001,16 l59.165. [31] ChironS, eta1. [J]. ChromatogrA, 2000,679 l37.145. [32] ChaoWS, JiangSJ. [J]. AnalAtSpectrom, 1996,13:1337.1341. [33] Guo Lei, Gui-Bin Jiang. [J]. Chemistry, 2001,7:456 * 459.082 pulsed-field gel electrophoresis and its application in the study pathogens Review Xiumei Li Yanjun revision (China Center for Disease Control and Prevention, Nutrition and Food Safety, Beijing 100021, China) Abstract: Pulse field gel electrophoresis in agarose gel electrophoresis, based on the orthogonal variable external electric field,[link widoczny dla zalogowanych], so that more than 50kb, or even more than a few thousand kb DNA molecule was also effective separation methods. The method used in molecular epidemiology, by observing the differences in electrophoretic bands in order to determine the genetic relationship between strains provides a reliable technological means. Has been widely used in tracing food-borne disease, virulence genes of pathogenic bacteria, hospital infection epidemic surveillance, research and other aspects of drug resistance, molecular epidemiology and the basic impetus to the development of science. Keywords: Electrophoresis; microorganisms; epidemiology CLC: Q93.33 Document code: A Article ID: 1001.1226 (2005) 05-0305 * 04 pulsed-field gel electrophoresis (pulse.fieldgelelectroph0resis, PFGE), also known as pulsed alternating field gel electrophoresis, the gel is applied orthogonal variable electric field, whenever a change in the direction of the electric field, more than 20kb of DNA molecules of the electric field along the new axis will redirect to adapt to the gel irregular pores changes, to continue DNA molecules are separated according to the scope of selecting the appropriate pulse time, if the DNA molecules change the direction of the electrical pulse cycle time is less than, DNA molecules can be separated according to their size. In recent years, PFGE and the application of the rapid development of widely used, has been involved in almost all the biological structure of the genome 1PFGE detection of pathogens in the application in principle because the bacteria are carried by the proliferation of the second, the Received Date: 2005.03 .02: Revised Date: 2005.06.30 Foundation: National Science and Technology, Reviewers Profile: Xiu-Mei Liu, female, researcher, research direction: food hygiene. Between the passage of bacteria with the same genetic material. The passage between the similarity between the bacterial genetic material is to identify common sources of pathogens excellent tool. In the past, infectious diseases are largely dependent on detailed evaluation of the epidemiological investigation and a number of positive cases were appropriate to determine the investigation of factors associated with specific diseases. Laboratory from suspected sources for the isolation and identification of pathogens to provide independent evidence of epidemiological assumptions. When the number of laboratory methods to identify species of bacteria to the following (serotype, biotype,[link widoczny dla zalogowanych], phage type), they can be put in the epidemiological investigation of suspicious cases and to match the source of infection. With the continuous development of molecular biology methods, from the level of DNA typing and identification of pathogenic has become possible. DNA large restriction fragment analysis with restriction enzyme digestion in a short genomic DNA, that would produce a small number of restriction fragments (usually l0 ~ 20 posts), and in accordance with the different fragments were identified on the DNA molecules . However, these fragments are usually too large, conventional agarose gel electrophoresis can not be separated. Because agarose gel electrophoresis for the average, in a certain voltage and agarose concentration, DNA migration rate is mainly related to its molecular size, namely,[link widoczny dla zalogowanych], the larger molecules, the slower migration rate, and when the linear DNA double helix radius More than gel pore size, the migration rate will not change,
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