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Low-frequency hearing loss WFS1 gene mutation screening in family


niatisTedS. Guide to molecular cloning experiments. Jin Dongyan, Li Mengfeng translation. 2. Beijing: Science Press, 1996.4634TakedaK. InoueH. TanizawaYeta1. , Ⅳ FS1 (Wolframsyndrome1) geneproduct: predominantsubcellularlocalizationtoendoplasmicretic-uluminculturedcellsandneuronalexpressioninratbrairLHumMolGenet, 2001,10 (5): 4775OsmanAA. SaitoM. MakepeaceCa1. Wolframinexpressionin-ducesnovelionchannelactivityinendoplasmicreticulummembranesandincreasesintracellularcaldum. JBiolChem, 2003,278 (52): 52755 (200410-10 Received 2005-02-19 Revised) (article editor Nan Feng) modified pericardiocentesis 38 cases Ningji deposit Lvfeng Ping Wang Guangyan An Xiuli March 2001 ~ December 2004 were treated in our hospital due to various reasons, patients with pericardial effusion 38 passengers, were performed in modified conventional pericardiocentesis. Preoperative two-dimensional ultrasound examination, patients take seats, severe illness who take the supine position. Avoid the myocardium, the partial paracentesis blunt apex anterior pericardial effusion below the distance to the wall, marked diastolic lcm of the outer edge of the pericardium visceral puncture point. Observation of the skin to the liquid section of the ultrasound probe and the skin depth and angle of the plane (the surgeon must visit the observation). Zhu Huanzhe surgery do not cough, breathe deeply, or to randomly change the position, good interpretation, the lifting of fear. ECG monitoring, monitoring of blood pressure, respiration, heart rate and rhythm. Patients were placed in position when the ultrasound examination, routine skin disinfection, sterile shop towel. Take 2 lidocaine local anesthesia. Hemostatic clamp off hose connected with the needle handle, prompted by ultrasonic rear puncture site is slow to stab people until there is a sense of frustration, then the needle of ultrasonic tips to the posterior wall of the pericardium from the anterior 3 / 5 supine position to the front wall of the pericardium on the lcm, and then the direction of the needle handle slightly tilted to the spine, keep the needle within the horseshoe-oriented, so as not to stick closed parietal pericardium difficulties caused by pumping liquid, fixed needle, take the syringe to extract fluid. Slow pumping liquid, while observing the patient breathing, blood pressure, heart rate and rhythm, feel free to ask the patient feels, so early detection of anomalies in time. Liquid pumping speed should be slow, for the first time when pumping liquid up to 100ml rest 5min, the heart has a process of adaptation. Aspiration side edge sword four chamber section ultrasound probe liquid segment, when the liquid para <1cnl stop pumping fluid around. Of surgery, closure of the needle point alcohol cotton ball, sterile dressing over fixed, submission specimens. This method is 38 times the puncture, are a successful, no complications. Has been avoided because of the direction of cardiac puncture, back piercing the pericardial cavity of the fluid, so to avoid the conventional precordial puncture inward, backward, to the spine; sternal puncture points up, after a little to the left until thorn, the risk of coronary myocardial injury; also avoid the large pericardial effusion increased liver congestion, liver injury Sternal puncture hazard. Conventional two puncture method, when pumped to the fluid margin is low, the systolic and diastolic, easy to hit the tip of coronary artery and myocardial scratch, this approach to the spine tilt the needle handle, depth of liquid in paragraph 3 / 5, systolic and diastolic hit the needle will not cause coronary artery and myocardial injury. By the above procedure 3 times before pumping fluid 100 ~ 300ml, heart rate fell by an average of about 1O times, before the patient feels more smooth breathing, heart palpitations, chest tightness symptoms reduced. 35 times after the section of fluid under the ultrasound tips lcm, patients with palpitations, chest tightness, symptoms of basic relief, up to a continuous pumping liquid 1300ml, no symptoms of acute myocardial expansion. 6h sharp increase in urine volume. Seat selection puncture point, depth of needle selection supine position, the lowest point for the pericardial effusion, fluid level section of the thickest, high success rate risk is small, pumping liquid more quickly alleviate symptoms. The improved cardiac puncture, simple operation without danger, safety, pumping liquid more quickly alleviate symptoms. (Received 2005-05-10 Revised 2005-02-04) (This edit Chen Juan) Author: People's Liberation Army 401 Hospital, Qingdao 266100, North Branch (Ningji deposit, Lvfeng Ping, Wang Guangyan, An Xiuli)