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Hebei Province in 2006 cases of acute flaccid paralysis surveillance of Virology


1), in which 26 strains detected in RD cells, the detection rate of 70.3%, L20B cells detected in 34 strains, the detection rate of 91.9%, indicating that the L20B cells on the sensitivity of poliovirus higher than RD cells. 2 PV cells in the case of combination of detection (Table 2), RD cells and L20B cell lines also detected PV24, accounting for 64.7% (24/37), L20B l3 separate strains detected in 35.3% (13 / 37), RD cells did not separate strain detected in PV, L20B cells with greatly improved the detection rate of PV. Table 12 kinds of cells in detection of polio virus cells in the table 22 the results of combined detection of polio virus in different cell 2.3.4 on the sensitivity of non-polio enteroviruses from stool specimens of 474 cases of AFP were detected NPEV64 strains, NPEV isolation rate was 13.5%. RD alone detected 58 (90.6%), L20B detection of a single (1.6%), 2 kinds of cells were also detected NPEV5 strains (7.8%). 3 Discussion virological monitoring in the AFP, WHO requires the use of L20B cells and RD cells 2, the former for the transfer of the limit of small PV cell receptor, and its introduction has played a rapid and specific separation of the role of PV. From 2006 on the polio virus in different cells results look, I / I) cells, undetected rate 29.7%, L20B cells missed was 8.1r, 2l9 that L20B cells more sensitive than PV RD cells,[link widoczny dla zalogowanych], L20B cells with greatly improved the detection rate of PV; and testing for NPEV, RD cells were isolated was 98.4% (63/64), L20B cell separation rate was 9.4% (6 / 64),[link widoczny dla zalogowanych], indicating that the sensitivity of RD cells NPEV higher than the L20B cells, the use of RD cells significantly increased the detection rate of NPEV. At the same time using two kinds of cells, can play a complementary role in the monitoring of pathogens in favor AFP. AFP surveillance system in Hebei Province for many years maintained a very high sensitivity, AFP reported incidence after 2000 were much more than 2.0/10 million,[link widoczny dla zalogowanych], virological monitoring the number of cases each year more than 300 patients in 2006 to 474 cases in Laboratory monitoring the case of extremely heavy workload of the monitoring indicators for many years maintained at a high level J. Polio laboratory in Hebei Province in 2006 once again, out of the functions of assessment results through, PV qualitative accuracy rate of 100%, not found wild poliovirus, the other monitoring indicators have reached the WHO request, show that the polio laboratory monitoring quality in Hebei Province ,[link widoczny dla zalogowanych], a well-functioning j. See the results, we must also face the problem of the existence of: �� the amount of stool samples and stool too much and too little spillover phenomenon exists in varying degrees. �� passing stool specimens collected on the classification of AFP cases is important], although the adoption of Hebei Province will pass rate of AFP cases has gradually increased and stabilized at 80% level, there are still a small number of AFP cases did not pass stool specimens collected. Stool specimen collection rate and in a timely manner and timely submission rate should be further improved. �� the completion of routine work should also conduct in-depth research, such as the identification of the type poliovirus, other enteroviruses further typing and so on. �� In the case of a large amount of samples, in order to maintain the level of quality monitoring, laboratory personnel, financial,[link widoczny dla zalogowanych], and material inputs should be increased accordingly. 4
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