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The quality of different manufacturers of thoracic Shun Qi Wan


. 09%; for the test sample and the average content of honokiol 0.520mg · g ~. RSD was 2.57%. 3.7 Stability Test to take the same control solution and test solution were measured at intervals of time sampling, a total study 30h, magnolol and honokiol reference substance, the test thoracotomy along Magnolia Qi Wan phenol peak and the peak area of ​​honokiol were less than 2% RSD, indicating that honokiol and magnolol in the 30h and the stability. 3.8 recovery test, accurately weighed samples of known content of 9 were,[link widoczny dla zalogowanych], respectively, a certain amount of magnolol and honokiol standard (in the form of the solution by adding the amount of 3 groups, play dry solvent), by determination method of measurement, calculation of recovery, the results shown in Table 1. Table 1 thoracotomy Shun Qi Wan (water pills) and magnolol and honokiol plus sample recovery test results of determination Tab1ResultofdeterminationforrecoveryofmagnololandhonokiolinKaixiongshunqipills3.9 control solution was prepared by the aforementioned test solution and were precise drawing of the two solutions 20, into the liquid chromatograph to measure and calculate the content. Results Table 2.4 4.1 Identification of the samples discussed according to Chinese Pharmacopoeia ... testing comply with the requirements established under the new TLC method to the detection of hesperidin and woody. 4.2 Determination of magnolol in this product and Honokiol Determination Although reported, but it is based on direct methanol extract of the test solution for, as the product of methanol extract of impurities, many times into the After the column like hypertension, which could easily lead to damage to the column, so the test solution preparation methods were studied. 4.2.1 Extracting and the conditions of magnolol and honokiol are the main active ingredients of Magnolia, for the selection of suitable extraction solvent, compared to the methanol, chloroform, ethyl acetate extraction, methanol extraction measured highest content of methanol as solvent to determine; The test compared extraction and reflux extraction ultrasound treatment no significant difference between the selected approach a simplified ultrasonic, and extraction time were investigated, no significant difference between the selected extraction time is 40min . Table 2 chest Shun Qi Wan (water pills) and magnolol and honokiol in the assay results Tab2ContentofmagnololandhonokiolinKaixiongshunqipills Note: The manufacturer name extract cloud after the change event of the mobile phase, with 90% methanol half the amount of dilution,[link widoczny dla zalogowanych], but also a large number of precipitation (turbidity), filtering more difficult. The test solution to reduce the pollution of the column, its purification,[link widoczny dla zalogowanych], the quantitative amount of methanol extracts of samples taken, evaporated after the dissolution by acidic water, add chloroform extraction, can be transferred most of the organic component under test layer, the acidic water layer was extracted twice and then chloroform extracted components can be tested fully, after the chloroform extract was concentrated, will be determined later, were not detected magnolol and honokiol peaks. Methanol extracts evaporated sample residue after the acidic solution, some can not be dissolved, the residual part can be extracted by the first turn with chloroform solution,[link widoczny dla zalogowanych], and then shaking extraction. The test solution 4.2.3 Selection of the Solvent extract samples of the chloroform evaporated, the residue, although soluble in methanol,[link widoczny dla zalogowanych], but methanol as the mobile phase polarity than large, with methanol as solvent in some of the test solution case of the smaller polar mobile phase composition appeared precipitation (turbidity), and the injection will result in the pollution of the column. Methanol or mobile phase with a water soluble residue, can avoid this phenomenon, then the remaining residue dissolved in methanol, magnolol and honokiol little content, can be ignored, selected with 90% methanol as solvent residues residue. To avoid the low 90% methanol at room temperature on solubility of residues, dissolved warm when appropriate. Manufacturers in 20 different qualitative identification of 23 batches of samples were positive, magnolol and honokiol content with the sum of the 0.148 ~ 4.962mg-g between the significant differences between levels, reflecting the existence of product quality quite different. Show that the increase in the standard item such determination is necessary.
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