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Neonatal Streptococcus pneumoniae isolates transposase gene genetic markers


, China) ABSTRACT: TofindouttheexistenceoftheconjugativetransposonsinStreptococcuspneumonoaeisolatedfromnewbornsinChangzhou, Jiangsuprovince,[link widoczny dla zalogowanych], thegeneticmarkerintTn916/Tn1545transposongenefrom47strainsofS. pneumoniaeisola'tedfromnewbornswasinvestigatedbyPCRamplification. Itwasfoundthat39stainsoutofthese47strainsofS. pneumoniae (83.O) showedtheexistenceofintTn916or/andintTn1545conjugativetransposon. Thehighprevalenceofthesetrans-posonsindicatesmanyantibioticsresistancegenesarefoundintheintTN916/intTN1545coniugativetransposon, suggestingthatS. pneumoniaecouldgainthemultipleantibioticsresistanceviathetransferoftransposons. KEYWORDS: newborn; Streptococcuspneumoniae; transposon; resistance; gene of Streptococcus pneumoniae (SrpcDcc Su, tetracycline,[link widoczny dla zalogowanych], cotrimoxazole and other antimicrobial drug resistance is rising, the Sp multi-drug resistant infections increased, so that treatment can be difficult. transposase gene and the spread of bacterial resistance genes are closely related. In order to understand Changzhou region isolated from neonatal respiratory bacteria isolated from Sp transposase enzyme genes, Present and Sp of 47 bacteria of transposable genetic marker gene - . 1 Materials and methods 1.1 Strains sources,[link widoczny dla zalogowanych], identification and susceptibility testing 47 Sp strains were from December 2003 to January 2006 was isolated from tracheal secretions in patients with neonatal respiratory tract infection specimens. 1h specimen collection of blood within the inoculated plates,[link widoczny dla zalogowanych], set of 5% CO. Incubator incubated 18 ~ 24h,[link widoczny dla zalogowanych], according to colony characteristics, microscopic morphology, Opp Tuoxin (Optochin) test evaluation. Standard strain ATCC49619 was cloned from the genetic Wuxi Institute of Technology as gifts. 47 Sp strains were reported by duPlessis other species-specific PCR detection confirmed that Sp (target gene pbp2B, primer sequences Pl5'-CTGACCATTGATTTGGCTTTCCAA a 3, P5 a TTTGCAATAGTTG (ACATACTG a 3, the purpose of the product length 682bp) sensitivity testing by agar dilution method, erythromycin susceptibility testing using K-B method according to NCCLS standards of interpretation. 47 is not sensitive to penicillin, which is not sensitive to erythromycin, 32 strains (erythromycin is not sensitive to rate of 68.1) . 1.2 strain DNA template preparation detected a single colony picked into lysis buffer containing 501 (0.5 non-ionic detergent prepared proteinase K solution concentration 200ng/ml) and 0.5m1 centrifuge tube, and placing 55. C water bath digestion 1h, 95 ℃ water bath set to change inactivated 10min. centrifugation 15000r/min30S. supernatant fluid is the template. 1.3 '-CATCAG Author: Child Health Hospital of Changzhou, Changzhou 213003Email: zhengyafengjby @ 126. corn