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Cholerny Spammer
Joined: 03 Mar 2011
Posts: 729
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Location: England
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 Posted: Sun 15:13, 20 Mar 2011 Post subject: adidas scarpe drs xcy lbg tvv |
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Streptomyces protoplast electrofusion
withahigh-yieldchlortetracycline (CTC) producerbyproto · P1asteIactrofusion. Theprotoplastsofthesecondparentstrain,[link widoczny dla zalogowanych], theCTCproducer. wereinactivatedbyheatbeforefusio1toatterupttoreducetheproportionoffusantswhichsynthesizedbothCTCandthedemethylateddcrlvatiyes. Twodifferenttypesofgenetransfersyst · ems, BAEKON2000andSDII,[link widoczny dla zalogowanych], weteemployedfornon-alternatingcurtentfieldeleetrofusionwithvariOUSparameters. butnofusionfrequencleswefehigherthanthatobserredincontrolexperimerits. TheairernatingcurrentfieIdelectrofusionwasconductedwithSD3000cellfusionapparatus. Theoptimumparameters (ACfield: stength800V/cm.frequencyO.5MHz, DCpalse: strength7kv/cm,[link widoczny dla zalogowanych], duratioll209s,[link widoczny dla zalogowanych], number3) resultedinfusantformationatfrequencyof2.0x10 one ,[link widoczny dla zalogowanych], whichwasaboutoneorderofmagnitudehighercomparlngwiththePEGindueedfusion. TheshakeflaskfermentatioilofthefasantsshowedthatthePere · eatageofhigh-tltrestrainsisolatedbyerossingtheDMCTCproducerwiththebeatinactivatedprotoplastsofCTCproducerwashigherthanthatofstrainsobtainedbyfusionbetweenthedivergeatauxotrophsofDMCTCprodueer. Inaddition. itwasfoundthatmorethan85fusantsfromDMCTCproducerxCTCproducer (inactivated) onlyprod · acedDMCTCDMTC. andtheotherssynthesizedCTCandTChcsidesthedemethylatedcomponents. Theseresultsimpliedthatforimpro · vementofproductivltyofoneantibioticproducer, itmightbensefultocrossitbyfusionwithinactivatedprotoplastsofanotherstrainwhichproducesacloselyrelatedantibioticofhighertitrc. KEYWORDSProtoplaste1ectr0fusionJre # tomycasaureofaciens) Demcthylchloroterracyeline · 168 ·
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