lin91957
Cholerny Spammer
Joined: 22 Jul 2010
Posts: 461
Read: 0 topics
Location: xgnddr
|
Posted: Sun 8:00, 20 Mar 2011 Post subject: 吡咯烷二硫 |
|
|
吡咯烷二硫氨基甲酸酯对大鼠胃缺血/再灌注损伤的影响
injuryinrats[J].CanJPhysiolPharmacol,2005,83(6):483—92.XiaHH,TalleyNJ.ApoptosisingastricepitheliuminducedbyHelicobacterpylofiinfection:implicationsingastricearcinogene—sis[J].AmJGastroenterol,2001,96(1):16—26.收稿Et期:2008—08—05修回日期:2008—09—08本文编辑:程春开超高分子聚乙烯颗粒对体外培养成骨细胞活性的影响刘林,许铁,秦宏敏,韩会峰(徐州医学院附属医院急救中心,江苏徐州221002)摘要:目的建立小鼠胚胎成骨细胞和超高分子聚乙烯(UHMWPE)颗粒的体外联合培养的新模型,初步研究超高分子聚乙烯颗粒对成骨细胞活性和核心结合因子1(Cbfcd)表达的影响。方法分为空白对照组和UH-MWPE颗粒干预组,2组常规培养小鼠胚胎成骨细胞,待细胞贴壁后装满培养基,UHMWPE颗粒干预组加入UH—MWPE颗粒,封闭倒置培养瓶,颗粒漂浮至贴壁细胞层。应用流式细胞技术分析各组细胞早期凋亡情况。分别在共培养的第3、5、7天检测细胞Cbfctl在mRNA和蛋白水平上的表达情况。结果封闭条件下小鼠胚胎成骨细胞正常生长可达10天;UHMWPE颗粒不影响细胞增殖活性,对细胞无明显毒性作用;UHMWPE颗粒干预组的目的基因Cbfcd表达增强。结论超高分子聚乙烯颗粒可以独立、直接影响成骨细胞相关因子的分泌。关键词:成骨细胞;超高分子聚乙烯颗粒;核心结合因子d1中图分类号:R687.4文献标志码:A文章编号:1000—2065(2008)09—0568—05Influenceofultra——highmolecularweightpolyethyleneonviabilityofosteoblastsLIULin,XUTie,QINHongmin,HANHuifeng(EmergencyCenter,AffiliatedHospitalofXuzhouMedicalCollege,Xuzhou,Jiangsu221002,China)Abstract:ObjectiveByestablishinganewmodeltoco—culturemouseembryoosteoblastsandultra—highmolee—ularweightpolyethylene(UHMWPE)particlesinvitrotostudytheeffectsofpolyethyleneonosteoblastactivityandrelat-edfactorsexpressioninvolvedinthemechanismofasepticlooseningofarthroprosthesis.MethodsTheexperimentwascarriedoutinablankcontrolgroupandaUHMWPEparticleinterventiongroup.Inthetwogroups,theembryonicmouseosteoblastswereconventionallycultured,withthecultureflaskfilledwithfluidmediumafterthecellsbeganadheringto基金项目:国家自然科学基金重点项目(50535050)$通信作者,E—mail:qinhongmin07@yahoo.com.cn
More articles related to topics:
复杂胫骨平台骨折38例治疗体会_3125
串联表达Fas shRNA腺病毒载体的构建_3732
[link widoczny dla zalogowanych]
The post has been approved 0 times
|
|